KOD DNA Polymerase
KOD DNA Polymerase* (formerly KOD HiFi DNA Polymerase) is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase (Nishioka 2001). KOD is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp with superior accuracy and yield (Takagi 1997). The enzyme’s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase.
The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than for Pfu DNA polymerase, resulting in highly accurate and robust yield, in a short reaction time. The enzyme generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.