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ACTaq™ Mastermix

Quick Overview

Product Description:

ACTaq™ Mastermix is complete “ready-to-go” 2X reaction mix which only requires consumer researcher to add water, template and primers. Taq DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75ºC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.

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Product Name Part # Image Price Qty
ACTaq™ Mastermix 2x, 50 reactions

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ACTaq™ Mastermix 2x, 200 reactions

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Additional Details

Dilution: 25μL of 2X ACTaq™ Mastermix is required for each 50μL reaction.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit incorporates 10nmol dNTP into acid-insoluble material in 30 minutes at 72ºC.

Product Composition: 2.5 Units of ACTaq™ DNA Polymerase, 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.