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ACTaq™ Hot-Start DNA Polymerase

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Product Description:

ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. ACTaq™ Hot-Start DNA Polymerase differs from ACTaq™ DNA Polymerase such that it contains antibody binding Taq which enables the activation of Taq only at 94oC. The activation step eliminates the presence of non-specifics such as primer-dimer formation and mis-primed products, by ensuring the enzyme is inactive at temperature below 94oC. ACTaq™ Hot-Start DNA Polymerase reduces risk of contamination, making reaction preparation at ease when carried out in room temperature.

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Product Name Part # Image Price Qty
ACTaq™ Hot-Start DNA Polymerase, 250U

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ACTaq™ Hot-Start DNA Polymerase, 1000U

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Additional Details

Hot-Start Activation Step: pre-incubate 2 minutes at 94oC prior to thermal cycling.

Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.

Concentration: 5 Units/μL

Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.

Product Components: ACTaq™ Hot-Start DNA Polymerase (Antibody bound recombinant Taq DNA Polymerase); PCR reaction buffer; 10X 25mM MgCl2. (Buffer and MgCl2 concentrations are customizable upon request)

Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.

PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM (NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.

Quality Control: ACTaq™ Hot-Start DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.