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ACTaq™ DNA Polymerase

Quick Overview

Product Description:

ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75oC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.

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Product Name Part # Image Price Qty
ACTaq™ Taq DNA Polymerase, 250U

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62-1047-1
$72.68
ACTaq™ Taq DNA Polymerase, 1000U

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62-1047-2
$230.74

Additional Details

Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.

Concentration: 5 Units/μL

Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.

Product Components: ACTaq™ DNA Polymerase, PCR reaction buffer, 10X 25mM MgCl2. (Buffer and MgCl2 concentrations are customizable upon request)

Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.

PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM (NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.

Quality Control: ACTaq™ DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.