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ACTaq™ Blue Mastermix

Quick Overview

Product Description:

ACTaq™ Blue / Red Mastermix is complete “ready-to-go” 2X reaction mix which only requires consumer researcher to add water, template and primers. ACTaq™ Blue / Red Mastermix differs from ACTaq™ Mastermix such that it contains blue / red dye added to the enzyme used for tracking and loading dye purposes, and therefore no loading buffer is required. Taq ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75ºC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.

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Product Name Part # Image Price Qty
ACTaq™ Blue Mastermix 2x, 50 rxns

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ACTaq™ Blue Mastermix 2x, 200 rxns

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Additional Details

Dilution: 25μL of 2X ACTaq™ Mastermix is required for each 50μL reaction.

Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.

Unit Definition: One unit incorporates 10nmol dNTP into acid-insoluble material in 30 minutes at 72ºC.

Product Composition: 2.5 Units of ACTaq™ Blue / Red Taq DNA Polymerase, 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.

Quality Control: ACTaq™ Blue / Red Taq DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.