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ACTaq™ Blue High Fidelity DNA Polymerase

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Product Description:

ACTaq™ High Fidelity DNA Polymerase, originating from Pyrococcus furiosus with molecular weight of 90KDa, is a thermo stable enzyme and exhibits the lower error rate of DNA amplification. For use in DNA fragment extension amplification reactions with 3' to 5' exonuclease proofreading activity; at extension amplification speed of 1000 bases per minute and optimized between 65-75oC, when using dNTP concentration at 100-300μM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.1. ACTaq™ High FidelityDNA Polymerase generates blunt-ended fragment. It comes with blue dye premixed with the enzyme used for tracking and loading dye purpose and therefore no additional loading buffer if needed.

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Product Name Part # Image Price Qty
ACTaq™ Blue Hgh Fidelity DNAPolymerase,250U

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ACTaq™ Blue Hgh Fidelity DNAPolymerase,1000U

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Additional Details

Storage Condition: -20oC. Stable for at least 12 months in constant freezer temperature.

Concentration: 1 Unit/μL

Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.

Product Components: ACTaq™ Blue High Fidelity DNA Polymerase, 10x PCR reaction buffer with Mg2+ (Buffer and MgCl2 concentrations are customizable upon request)

Storage Buffer: 10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol.

PCR Reaction Buffer: 10x PCR buffer with Mg2+. 20mM MgSO4, 100mM KCl, 160mM (NH4)2SO4, , 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.

Quality Control: Activity of PCR for 1Kb fragment. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.